ABSTRACT
Objective: To investigate the induction effect of oxymatrine on the apoptosis process in rat hepatic stellate cell line HSC-T6 and to define its impact on telomerase activity and mRNA expression of subunit telomerase reverse transcriptase (rTERT). Methods: HSC-T6 cells were cultivated with different concentration of oxymatrine for different time periods. Effect of oxymatrine on the growth inhibition of HSC-T6 cells was analyzed by MTT assay. Apoptosis of HSC-T6 cells was detected by flow cytometry. Telomerase activity was determined by TRAP-PAGE-silver staining and the expression of rTERT mRNA was examined by RT-PCR assay. Results: Oxymatrine significantly suppressed the growth of HSC-T6 cells and induced apoptosis, and also reduced the activity of telomerase and inhibited the rTERT-mRNA expression in HSC-T6 cells. Conclusion: The function that oxymatrine inhibits the proliferation of HSC-T6 cells may be associated with its action on the cellular telomerase and telomerase rTERT-mRNA activity.
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of Notoginseng Radix on hepatic expression of transforming growth factor beta1 (TGF-beta1) and connective tissue growth factor (CTGF) in rats with alcoholic liver disease (ALD), in order to discuss its protective effect on alcoholic cirrhosis.</p><p><b>METHOD</b>Fifty SD male rats were divided into the normal control group, the model group, the high-dose and low-dose Notoginseng Radix groups (3.0, 12.0 g x kg(-1)) and the magnesium isoglycyrrhizinate group (24 mg x kg(-1)), with 10 rats in each group. Apart from the control group, other groups were administered with ethanol-cornoil-pyrazole for 14 weeks to establish the alcoholic liver disease model. During the establishment of the model, the high-dose and low-dose Notoginseng Radix groups were administered with 12 g x kg(-1) x d(-1) Notoginseng Radix for 14 weeks, once everyday. Efforts were made to detect liver function, pathology with Masson staining, and the expressions of TGF-beta1, Smad3, Smad7 and CTGF mRNA.</p><p><b>RESULT</b>Compared with the rats in model group, rats in Notoginseng Radix groups showed significant reduction in liver ALT, AST, collagen fiber deposition, and TGF-beta1, Smad3 and CTGF mRNA expressions in liver tissues, with the increase in the expression quantity of Smad7 mRNA. There were differences between the Notoginseng Radix groups. No significant difference was observed between the high-dose Notoginseng Radix group and the magnesium isoglycyrrhizinate group.</p><p><b>CONCLUSION</b>Notoginseng Radix can affect TGF-beta1/Smads signaling pathway and reduce the expression of CTGF.</p>
Subject(s)
Animals , Humans , Male , Rats , Connective Tissue Growth Factor , Genetics , Metabolism , Drugs, Chinese Herbal , Gene Expression , Liver Diseases, Alcoholic , Drug Therapy , Genetics , Metabolism , Panax notoginseng , Chemistry , Rats, Sprague-Dawley , Smad3 Protein , Genetics , Metabolism , Smad7 Protein , Genetics , Metabolism , Transforming Growth Factor beta1 , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To determine the reversal effect of aloe emodin liposomes (AE-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP.</p><p><b>METHOD</b>The un-cytotoxic and cytotoxic concentration of AE-L, and un-cytotoxic concentration of E-L were determined by MTT. The sensitivity of cisplatin were determined by MTT assay in above 3 groups. The intracellular concentration of cisplatin was detected by inductively coupled plasma mass spectrometry (ICP-MS).</p><p><b>RESULT</b>The maximum non-toxic concentration group of AE-L (2.0 mg x L(-1)) increased the sensitivity of cisplatin in A549/DDP, decreased IC50 of cisplatin in A549/DDP from 16.81 mg x L(-1) to 5.86 mg x L(-1), and the hyp-cytotoxic concentration (7.0 mg x L(-1) ) group's IC50 decreased to 4.34 mg x L(-1); AE-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells.</p><p><b>CONCLUSION</b>The results showed that aloe emodin can reverse multidrug resistance (MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.</p>